Disinfection of drinking water inactivates (i.e., kills or prevents pathogens from replicating) waterborne pathogens, such as bacteria and viruses. The amount of microorganisms needed to cause an infection varies widely between pathogens. The median infectious dose for Salmonella typhosa is 1,000,000 organisms (but can be much less for infants), Hepatitis A virus is between 10-100, and Giardia lamblia and Cryptosporidium are less than 10 organisms. Disinfection does not completely eliminate all living organisms in the water, but does significantly reduce potential acute waterborne disease risk. The pathogen risk reduction is expressed in terms of “log inactivation”. For example, a 3.0 log inactivation value means that 99.9% of microorganisms of interest are inactivated.
Disinfection can be accomplished using either chemical oxidants, such as free chlorine, chloramines, or ozone, or by photo-inactivation with ultraviolet light. Most Colorado water systems use free chlorine as their main disinfectant. Disinfection with chlorine or chloramines provides a persistent disinfectant residual in the distribution system. This disinfectant residual can provide defense against harmful organisms that enter the distribution system through backflow events, pipeline leaks, low pressure events, or other contamination pathways.
Regulation 11: Colorado Primary Drinking Water Regulations requires that systems maintain a minimum disinfectant residual concentration of 0.2 mg/L at the entry point and 0.2 mg/L in the distribution system. These two requirements are the same for groundwater (GW), groundwater under the direct influence of surface water (GWUDI), and surface water (SW) systems. Each source type has additional disinfection requirements outlined below:
- GW sources are considered protected groundwater and are expected to have limited or no pathogens. GW systems must comply with the Groundwater Rule through either triggered source water monitoring in the event of a total coliform positive sample or by certifying that the GW treatment process always provides 4-log (99.99%) inactivation of viruses.
- GWUDI and SW sources likely have pathogen sources in the water (e.g., beavers, point discharges). Pathogenic organisms are expected and GWUDI/SW systems must meet pathogen log removal/inactivation requirements in the Surface Water Treatment Rule (SWTR). The required treatment is based on three target pathogens:
- Cryptosporidium: 2-log (99%) removal.
- Higher levels may be required based on LT2 source water monitoring.
- Giardia lamblia: 3-log (99.9%) removal/inactivation.
- Viruses: 4-log (99.99%) removal/inactivation.
For SW/GWUDI systems: filtration and disinfection are two complementary critical barriers for protecting public health. Drinking Water Policy 4 outlines the removal credits for various filtration types (e.g., conventional filtration, direct filtration). The disinfection treatment must be designed and operated to provide any remaining log inactivation required in the SWTR. These processes work together to ensure that drinking water is safe for the public.
Next time, we will discuss disinfection log inactivation and the critical parameters for design and compliance demonstration.
Below are some resources that will give more specific information about the requirements outlined above. If you have questions, please contact Melanie Criswell at melanie.criswell@state.co.us.
Resources
- Quick guide: Disinfectant residual
- Quick guide: Minimum disinfection for groundwater
- Drinking Water Policy 4 (DW004): Surface water treatment rule performance assessment, proper operation and fostering improved operations
- Drinking Water Policy 4 - Policy guidance
- Additional information about disinfection is available at the division's Revised Total Coliform Website
➽ Melanie Criswell - Lead Service Line, Corrosion, and Emerging Contaminants Engineer






